Bilirubin Direct

SIM

10510 

CPT

82248 

Synonyms

D Bili; D Bilirubin; Neonatal Bilirubin 

Specimen Type

Plasma or Serum 

Container

3.5 ml Green - Lithium heparin plasma separator 
4.0 ml Gold-top serum separator

Collection Instructions

None

Volume

2 ml plasma or serum 
Min: 0.5 ml

Additional Information

none

Patient Preparation

none

Specimen Processing Instructions

Plasma:  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes

Serum:  Allow to clot.  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes

Centrifuge within 2 hours of collection

Transport Requirements

Refrigerated

Specimen Stability

Room temperature:  8 hours   Refrigerated: 5 days 

Rejection Criteria

•  If not centrifuged within 2 hours of collection. 
•  Gross Hemolysis

Reference Range

0.0 – 0.2 mg/dl

Critical Value

none 

Test Includes

Direct Bilirubin 

Methodology

Timed Endpoint Diazo method 

Testing Frequency

Daily, Monday - Sunday 

Reviewed By

Crystal Killian

Reviewed Date

3/17/2017

Supplemental