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Lactic Acid or Lactic Acid Reflex


11600 = Lactic Acid and 11610 = Lactic Acid Reflex 





Specimen Type



2.0 ml Gray – Sodium Fluoride Potassium Oxalate Plasma

Collection Instructions

Draw to dark line on tube, 2.0 ml. 
Place on ice after collection, Centrifuge for 5 minutes, separate from contact with cells within 15 minutes of collection. 


1 ml plasma 
Min: 0.5 ml 

Additional Information

Plasma should be physically separated from contact with cells within 15 minutes of sample collection and analyzed without delay.   If sample cannot be tested immediately after centrifuging, pipet plasma from the cells, place in a tube with a cap, cap tightly, refrigerate up to 14 days.  FOR CSF samples only, samples are stable stored at 2-8 degree Celsius for up to 24 hours. 
IF Lactic Acid Reflex is greater than or equal to 2.0 mmol/L, then another Lactic Acid will automatically be ordered for collection 3 hours later.

Patient Preparation


Specimen Processing Instructions

Plasma:  Centrifuge at 3380 rpm’s (±100) for a minimum of 5 minutes

Separate from contact with cells within 15 minutes of sample collection and analyzed without delay.

Transport Requirements

On Ice or in an ice pack

Specimen Stability

15 minutes 

Rejection Criteria

Gross Hemolysis

Reference Range

0.3 – 2.1  mmol/L

Critical Value

Greater than 4.0 mmol/L 

Test Includes

Lactic Acid 


Measuring the absorbance due to the chromophore using an endpoint technique 

Testing Frequency

Daily, Monday - Sunday 


Reviewed By

Sandy Hammett 

Reviewed Date



If Lactic Acid Reflex is ordered, if the results are  greater than or equal to 2.0, our LIS will automatically order a Lactic Acid to be collected 3 hours after the collection time.

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