Skip to Content

View Additional Section Content

Leukocyte Alkaline Phosphatase (LAP) *

SIM

10861 Lab Corp Misc. 

CPT

85540 

Synonyms

LAP;  Neutrophil Alkaline Phosphatase 

Specimen Type

Whole Blood - ONLY USE GREEN HEPRANIZED TUBES WITHOUT GEL.  Send 6 freshly made blood smears, not stained. 

Container

4.0 ml Green - Lithium heparin whole blood without a gel separator. 
Slides

Collection Instructions

Prepare slides on 6 frosted end slides made from fingerstick blood or immediately after drawing in heparin tube. Air dry slides and label with patient name. Do not fix slides.

Volume

2 ml Whole Blood 
Min: 1.0 ml 
Six smears/ Minimum 2 smears 

Additional Information

Aids in the differential diagnosis of chronic myelocytic leukemia (CML) versus leukemoid reaction.

Patient Preparation

None

Specimen Processing Instructions

None

Fresh Whole Blood is needed.

Transport Requirements

Smears can be maintained at room temperature for 7 days

Specimen Stability

Fresh Whole Blood is the optimal specimen choice. 

Rejection Criteria

•  Collection in gel separator tube or slides made from any anticoagulant  other than heparin 
•  Clotted sample 
•  Frozen Slides 
•  Slides more than 7 days old 

Reference Range

Reference range accompanies report

Critical Value

 

Test Includes

LAP smears  

Methodology

Naphthol AS-B1 phosphate and freshly prepared fast red violet LB salt or fast blue BB salt buffered at pH 9.5 with 2-amino-2 methyl –1, -3-propanediol (AMPD) stain.  One hundred cells are counted and score totaled.  

Testing Frequency

 

LCA

001966 

Reviewed By

Teresa Price

Reviewed Date

2/20/2018

Supplemental

*  Reference Lab test

Was this page helpful?

Yes
No

Thank you for your feedback!

Thank you for your feedback and helping us to improve our website.
There will be no additional response.