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Methotrexate

SIM

50205 

CPT

80299 

Synonyms

MTX 

Specimen Type

Serum 

Container

4.0 ml Gold-top serum separator

Collection Instructions

None

Volume

2 ml serum, minimum 0.5 ml

Additional Information

Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA).  HAMA present in serum may interfere in this assay.  These samples should not be assayed with the MTX assay.

Patient Preparation

none

Specimen Processing Instructions

Serum:  Allow to clot.  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes

Centrifuge within 2 hours of collection.

Protect from Light if not stored in a dark refrigerated.

Transport Requirements

Refrigerated

Specimen Stability

Room temperature:  8 hours   Refrigerated: 24 hours 

Rejection Criteria

If not centrifuged within 2 hours of collection.

Reference Range

NO NORMAL VALUES.

Lowest reportable = less than 0.2 umol/L

Critical Value

none 

Test Includes

Methotrexate 

Methodology

Enzyme Immunoassay 

Testing Frequency

Daily, Monday - Sunday 

 

Reviewed By

Sandy Hammett 

Reviewed Date

2/19/2018 

Supplemental

 

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