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PTH (Parathyroid Hormone) with Calcium




83970; 82310 


PTH w/Ca 

Specimen Type

Serum and or plasma 


PTH:  4.0 ml Gold-top serum separator or 
         3.0 ml EDTA lavender stopper tube (EDTA is for PTH only) 
Calcium:  3.5 ml Green - Lithium heparin plasma separator or 
               4.0 ml Gold-top serum separator 

Collection Instructions



2 ml serum or plasma, minimum 0.5 ml

Additional Information

PTH:  Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays.  Patients routinely exposed to animals or to animal serum products can be prone to this interference and anomalous values may be observed.  Additional information may be required for diagnosis.

Patient Preparation


Specimen Processing Instructions

Serum:  Allow to clot.  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes

Plasma:  Centrifuge at 3380 rpm’s (±100) for a minimum of 10 minutes

Centrifuge within 2 hours of collection.

Do Not freeze serum or plasma for the PTH

Transport Requirements


Specimen Stability

PTH:  Room temperature:  4 hours   Refrigerated: 48 hours   It is also extremely important to ensure that patient samples have beenhandled and stored correctly.  Incorrect handling of samples will result in a lost of intact PTH.  

Rejection Criteria

If not centrifuged within 2 hours of collection. 
Frozen serum or plasma 

Reference Range

PTH:  19-88 pg/ml

Calcium:  0-16 yrs = 8.5 – 11.5 mg/dl

                 17 yrs and up = 8.9 -10.3 mg/dl

Critical Value

PTH = None;  Calcium = <6 mg/dl and >13 mg/dl 

Test Includes

PTH and Calcium 


PTH:  Chemiluminescent technology;  Calcium:  Indirect Potentiometry utilizing a Calcium ion selective electrode in conjunction with a Sodium reference electrode.  

Testing Frequency

Daily, Monday - Sunday 


Reviewed By

Sandy Hammett 

Reviewed Date




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