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Serotonin Release Assay *

SIM

20500 

CPT

82542 

Synonyms

SRA; Functional HIT Assay; Heparin -dependent Platelet Antibody

Specimen Type

Serum 

Container

Red-top or gold-top tube

Collection Instructions

None

Volume

Volume: 1 mL
Minimum volume: 0.5 mL 

Additional Information

Confirmatory assay used in the evaluation of heparin induced thrombocytopenia. 

Patient Preparation

Specimen Processing Instructions

 Once in the lab,separate serum from cells, transfer serum to a plastic transfer tube and freeze ASAP

Transport Requirements

Frozen

Specimen Stability

Frozen: 6 months 

Rejection Criteria

Plasma specimen; specimen not received frozen; grossly hemolyzed, icteric and lipemic specimens.

Reference Range

Reference range accompanies report

Critical Value

 

Test Includes

Serotonin Release Assay  

Methodology

Detects activation of washed donor platelets by measuring the endogenous serotonin induced by addition of patient serum. The serotonin levels are measured by highly sensitive liquid chromatography/tandem mass spectroscopy method. Therapeutic levels of heparin in the assay mix allow for the formation of PF4/heparin complexes on the donor platelet surface to provide an antigen target for the HIT antibodies. The specificity of the assay is increase by assessing the inhibition of platelet activation by adding heparin in excess, thus providing the heparin dependency of the serotonin release.

Testing Frequency

 

LCA

150018 

Reviewed By

Lisa McBride             

Reviewed Date

2/20/2018

Supplemental

* Denotes Reference Lab Test

Reviewed by Lisa McBride on February 20, 2018

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