Cortisol, Urinary Free (24 hour) *

SIM

LAB382

CPT

82530

Synonyms

Cortisol,24-hour Urine 

Specimen Type

Urine 24-hour 

Container

24-hour plastic urine container with no preservative. It is acceptable to collect cortisol with boric acid or HCl preservative.

Collection Instructions

Instruct the patient to void at 8 A.M. and discard the specimen. Then collect all urine including the final specimen voided at the end of the 24 hour period. (ie,8 A.M. the next morning) Container must be labeled with the patient name, date and time collection started and date and time collection finished .Refrigerate during the collection. Screw lid on securely.

Volume

Volume: 100 ml. aliquot 
Min. volume: 10 ml. aliquot 

Additional Information

Useful in the initial evaluation of patients with suspected Cushing syndrome.

Patient Preparation

Specimen Processing Instructions

Mix urine well. 
Measure and aliquot the 24 hour volume. 
Write the total 24 hour volume on the aliquot container.

Transport Requirements

Room Temperature

Specimen Stability

Room Temperature, Refrigerated, or Frozen for 14 days 

Rejection Criteria

Reference Range

Reference Range accompanies report

Critical Value

Test Includes

Free Cortisol 

Methodology

Liquid chromatography/tandem mass spectrometry (LC/MS-MS) 

Testing Frequency

LCA

004432 

Reviewed By

Rhonda Crowe

Reviewed Date

2/22/23

Supplemental

* Denotes Reference Lab test