Direct Coombs

SIM

LAB274

CPT

86880 

Synonyms

Coombs-Direct, Direct Antiglobulin Test (DAT) 

Specimen Type

Whole Blood, Plasma, Serum, or Cord Blood (Neonates only) 

Container

6.0 ml Pink – EDTA (preferred) 
4.0 ml Lavender – EDTA 
Neonates:  EDTA cord blood or 500 ul lavender microtainer 

Collection Instructions

None

Volume

Max:  6.0 ml whole blood EDTA 
Min:  Adults - 2.0 ml whole blood EDTA 
        Neonates* – EDTA cord blood specimen or 500 ul 
                           lavender microtainer 

Additional Information

None

Patient Preparation

None

Specimen Processing Instructions

None

Transport Requirements

Refrigerated

Specimen Stability

3 Days 

Rejection Criteria

Missing patient identification

Reference Range

Negative

Critical Value

N/A 

Test Includes

Direct Coombs.  If positive and the patient has been transfused or pregnant within the past 3 months an elution and eluate identification shall be performed unless already resulted in past 30 days. 

Methodology

Solid Phase, Column Agglutination Technology, or Tube method  

Testing Frequency

Daily, Monday-Sunday 

Reviewed By

Michele Earley 

Reviewed Date

3/22/23

Supplemental