Factor V

SIM

20130 

CPT

85220 

Synonyms

Specimen Type

Sodium Citrate Plasma 

Container

Two tubes – 4.5 ml Sodium Citrate 3.2% Blue stopper tube

Collection Instructions

None

Volume

4 ml plasma 
Min: 2.0 ml

Additional Information

If Plasma is removed, must be stored in Plastic tubes.

Patient Preparation

none

Specimen Processing Instructions

Whole Blood: send the collection tube

Plasma: Centrifuge at 3380 rpm’s (±100) for 5 minutes. Remove the plasma without disturbing the cellular layer. Centrifuge again at 3380 rpm's (±100) for 5 minutes. Remove the platelet poor plasma to a transfer tube. Freeze sample to extend stability. 

Transport Requirements

Refrigerated if within 4 hours

If transport time to lab exceeds 4 hours send frozen.

Specimen Stability

Refrigerated Whole Blood: 4 hours;  

Frozen Plasma: 1 month 

Rejection Criteria

•  Clotted Sample 
•  Insufficient volume 
•  Hemolyzed Plasma

Reference Range

Factor V  = 61 - 137 % activity.

Critical Value

None 

Test Includes

Factor V 

Methodology

Electromagnetic mechanical clot detection system 

Testing Frequency

7 a.m. - 7 p.m. only, Daily, Monday - Sunday 

Reviewed By

Crystal Killian

Reviewed Date

3/18/2021

Supplemental